Measuring soil respiration is one of the most sensitive indicators affecting soil quality and efficiency. Therefore, with the aim of investigating the effect of separate and integrated systems of urea, cow manure and poultry manure on soil CO2 emissions and estimating carbon mineralization potential as well as sorghum dry matter production, a randomized complete block design with 4 replications was conducted in the research field. Shahrekord University was established in 2010. Treatments included no fertilizer (control), separate application of urea, bovine and poultry manure (as chemical and organic sources of nitrogen, respectively) and three combined 50:50 compounds (urea + bovine, urea + poultry, bovine + poultry). The results showed that the highest cumulative soil respiration rate (141.39 g / m2) and respiration rate (3.8 g / m2 / day) were obtained by using poultry manure and the lowest soil respiration rate was related to the control treatment. In separate poultry manure treatment, the highest amount of sorghum dry matter (11.16 tons per hectare) was observed, but the dry matter produced in this treatment was not significantly different from treatments containing urea. Carbon mineralization potential was not affected by fertilization. In general, poultry manure with more nutrients compared to other fertilizer sources, in addition to beneficial effects on soil respiration, increases the dry matter of sorghum. It can also reduce the risk of environmental pollution by replacing chemical fertilizers in mulberry cultivation.